VecFashion allows you to purchase customized vectors in any one of these three ways. VecFashion is an easy and intuitive online tool that allows you to create vectors. Click on Design My Vector to get started. Follow the steps from there. You can create almost any vector you like (e.g. regular plasmid, lentivirus, adenovirus, overexpression, shRNA, gRNA, etc.) It takes just a few mouse clicks and can be done in minutes. You can send us a request for design if you are unable to create the vector you desire. We will create it for free. To find the gene that interests you, you can browse our selection of vectors by clicking the button Search for custom vectors at our top. Below are details about the design process.
Making your vectors usingVecFashion
Step 1
To be taken to the Select Vector Systems page, click Create My Vector from the VecFashion homepage. You’ll see a wide range of vector systems. You can then click the “Go to Design” button next to the option you choose. If you want to create a lentiviral vector to express your desired gene in mammalian cells then click on the “Go to Design” button next to Lentivirus under the Mammalian Gen Expression Vectors section.
You can find all technical information for each of the vector systems on our Guide on Vector Systems Page.
Step 2
You’ll see maps for the chosen vector system. The map shows two types of components. There are two types of components on the map. You can also add custom components to the backbone. You can add individual components to the backbone. Other elements such as the marker that detects drug or the fluorescent reporter are also possible. You have two options when adding a component. One is to select it from the database, and the other is to copy and paste the sequence. You can insert epitope tags and modify an ORF if you’re adding it. These are some tips:
Selecting components from a Database:
We have two types of sequences in our extensive component database. The first is the most popular components. These are available in an option that you can choose from (e.g. Popular promoters include CMV, EF1A and CAG. You can also search databases that are based on ORFs shRNAs and gRNAs. These databases allow you to search for specific genes and species and locate the component that is related to the gene you are looking for. If you want to create a vector that contains BMP2 from humans, then select an ORF database from the drop-down menu and search for human BMP2. You should see a complete list of RefSeq transcripts for this gene, which may include one suggested by VecFashion. You can then select an ORF from one of these transcripts and add it into your vector. Your ORF sequence can be modified (e.g. Before including the vector, you can insert epitope tags or add mutations.
All our well-known components can be found on the Guide to Vector Components Page.
ORF editing capabilities: Insert epitope tags or introduce point mutations to your ORF. After you have already added the ORF to your vector, it is possible to do so. Click on the title of the ORF in the vector map and then click Edit ORF from the menu drop-down. You can modify sequences by opening an editor. Click on the “Add tag button” under your sequence to open an editor that will display a list of tags to be added to the N or C ends in your ORF. You can also edit your sequence by entering or deleting nucleotides. You can edit any component you have downloaded from the database. Every edit will be recorded to show the changes to the reference sequence. In the sequence editor window, there are other handy functions for you to use, such as translating nucleotide sequences into amino acid sequences, finding ORFs, and showing the sequence of the reverse-complementary strand.
Putting multiple ORFs on a single promoter
When creating an expression vector for a gene you can place up to four ORFs behind the promoter, creating a polycistronic. Multiple ORFs may be expressed individually or as separate proteins using IRES linkers, 2A, or as fusion proteins. The Fashion Design Templates Studio window has an option called “Choose the number of ORFs”. This allows you to specify how many ORFs will be on your promoter.
Step 3
After you are done creating your vector, click Finish the Design. You will then be taken to the Page for Vector Information. You will see the vector’s detailed map and patterns. It is then possible to add it to your cart, store it online and send it out to your friends. You can also download the entire summary of the design in PDF format. This includes publications-ready images and GenBank format.
We need to create the vectors that you require for your.
VecFashion can sometimes be difficult to use. For instance, VecFashion may require an extra backbone. To send your vector details, simply go to VecFashion’s homepage and click “Send design request”. No cost to you! Our highly-trained engineers will design it and provide details about the price and turnaround time for when you request cloning.
We scan our vectors to identify the gene that you are looking for.
You can also search for popular vector designs matching your gene by clicking Search for Popular Vectors. This feature will display a list common graphic designs used in gene expression and knockdowns using basic backbones. This option allows you to check if we have ready-made vectors for the gene that interests you. You can click on the desired one to view its entire annotation sequence and map. Then, you can make any changes to the design if necessary.
